During the workshop, there will be ample opportunity for discussions with the instructors. Background: In the neurosciences, the physical disector method represents an established quantitative stereological method for unbiased sampling and counting of cells in histological tissue sections of known thickness. Participants should bring their specific projects and questions. The physical disector method, an alternative to the optical disector, is considered an effective and unbiased tool for estimating neuron numbers (Popken and Farel, 1996). Application of the physical disector principle for quantification of dopaminergic neuronal loss in. light microscopy, confocal laser scanning microscopy, multi-photon microscopy, electron microscopy) as well as non-invasive scanning techniques like CT and NMR are welcome. The most important feature of stereological approaches is that they are independent of assumptions that may lead to systematic deviation from real values. Fabricius K., Barkholt P., Jelsing J., Hansen H.H. Applications involving any kind of microscopy (e.g. This workshop is intended for students and researchers from the field of biology and medicine interested in sampling and measurement of 3D structures. The workshop encompasses lectures, classroom exercises with real biomedical images, and laboratory practicals demonstrating the implementation of proper sampling and processing techniques on real biological organs. It estimates the number, length, area, and volume of. Experimental design, sampling, use of stereological probes, estimation of stereological parameters, and the application of statistical methods appropriate for stereology are covered. Unbiased stereology is recognized in biological research as the best-practice method for quantifying biological features in tissue sections. This workshop provides an introduction to state-of-the-art stereology with emphasis on its practical application in biomedicine.